MicroRNA-223 Suppresses the Canonical NF-kappaB Pathway in Basal Keratinocytes to Dampen Neutrophilic Inflammation.
Zhou W, Pal AS, Hsu AY, Gurol T, Zhu X, Wirbisky-Hershberger SE, Freeman JL, Kasinski AL, Deng Q
Cell Rep. 2018 Feb 13;22(7):1810-1823. doi: 10.1016/j.celrep.2018.01.058.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 97142 | miR-223-RFP pCDNA | expression of miR-223 and TagRFP |
| 97143 | miR-vector-RFP pCDNA | for expression of miRNA and TagRFP |
| 97144 | Tol2-lyzC-Gal4-Vp16-Crys-CFP | For Gal4/UAS expression system |
| 97145 | Tol2-UAS-Dendra2 | For Gal4/UAS expression system |
| 97146 | Tol2-UAS-Dendra2-miR-223 sponge | For miR-223 knock down |
| 97147 | Tol2-lyzC-RFP-miR-30 backbone | As a control of miRNA expression |
| 97148 | Tol2-lyzC-RFP-miR-223 | miR-223 expression |
| 97149 | Tol2-krt4-RFP | As a control of miRNA sponge |
| 97150 | Tol2-krt4-RFP-miR-223 sponge | For miR-223 knock down |
| 97151 | Tol2-krt4-GFP | label apical epithelial cells in zebrafish |
| 97152 | pSI-check2-Cul1a-3'UTR | For dual luciferase report assay |
| 97153 | pSI-check2-Cul1b-3'UTR | For dual luciferase report assay |
| 97154 | pSI-check2-Traf6-3'UTR | For dual luciferase report assay |
| 97155 | pSI-check2-Tab1-3'UTR | For dual luciferase report assay |
| 97156 | pSI-check2-CUL1-3'UTR | For dual luciferase report assay |
| 97157 | pSI-check2-TAB2-3'UTR | For dual luciferase report assay |
| 106979 | pSI-Check2-hRluc-NFkB-firefly | Luciferase reporter with internal control for NFkB signal. |