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Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform.

Rock JM, Hopkins FF, Chavez A, Diallo M, Chase MR, Gerrick ER, Pritchard JR, Church GM, Rubin EJ, Sassetti CM, Schnappinger D, Fortune SM
Nat Microbiol. 2017 Feb 6;2:16274. doi: 10.1038/nmicrobiol.2016.274. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115162PLJR962Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis
115163PLJR965Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Antibodies from Article