Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

Parallel Genomic Engineering of Two Drosophila Genes Using Orthogonal attB/attP Sites.

Blanco-Redondo B, Langenhan T
G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 10.1534/g3.118.200565. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115158pHD-mW-attPCC-FRT (pTL717)Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.
115159pGE-attBCC-FRT-mW (pTL788)Integration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.
115160pGE-attBTT-loxP-DsRed (pTL780)Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

Antibodies from Article