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Parallel Genomic Engineering of Two Drosophila Genes Using Orthogonal attB/attP Sites.

Blanco-Redondo B, Langenhan T
G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 10.1534/g3.118.200565. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115158pHD-mW-attPCC-FRT (pTL717)Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.
115159pGE-attBCC-FRT-mW (pTL788)Integration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.
115160pGE-attBTT-loxP-DsRed (pTL780)Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

Antibodies from Article