mTORC1 Controls Phase Separation and the Biophysical Properties of the Cytoplasm by Tuning Crowding.
Delarue M, Brittingham GP, Pfeffer S, Surovtsev IV, Pinglay S, Kennedy KJ, Schaffer M, Gutierrez JI, Sang D, Poterewicz G, Chung JK, Plitzko JM, Groves JT, Jacobs-Wagner C, Engel BD, Holt LJ
Cell. 2018 Jul 12;174(2):338-349.e20. doi: 10.1016/j.cell.2018.05.042. Epub 2018 Jun 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 116928 | pAV106-pTDH3-mCherry-10xSumo-6xSIM | Yeast integrative vector for expression of the SUMO-SIM complex |
| 116930 | pRS305-Leu2-PINO4-PfV-GS-Sapphire | Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter |
| 116931 | pRS306-URA3-PHIS3-AqLumSynth –Sapphire | Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter |
| 116932 | pRS306-URA3-PHIS3-PfV-GS-Sapphire | Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter |
| 116933 | pCDNA3.1-pCMV-PfV-GS-Sapphire | Mammalian vector for expression of the 40nm GEM |
| 116934 | pCMV-PfV-Sapphire-IRES-DSRed | Mammalian lenti vector for expression of the 40nm GEM and DSRed |
| 116935 | pRS306-PHIS3-GFP-μNS | Yeast integrative vector for the expression of μNS |
| 116936 | pRS306-pHIS3-2xGFP-tURA3 | Yeast integrative vector for the expression of 2xGFP |
| 116937 | pT7-HISx6-SUMOx10 | Bacterial expression vector for 10 sumo |
| 116938 | pT7-HISx6-SIMx6 | Bacterial expression vector for 6 sim |