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Phosphorylation of Atg9 regulates movement to the phagophore assembly site and the rate of autophagosome formation.

Feng Y, Backues SK, Baba M, Heo JM, Harper JW, Klionsky DJ
Autophagy. 2016;12(4):648-58. doi: 10.1080/15548627.2016.1157237. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
114667pRS406 ATG9-GFP S122DExpresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag
114668pRS406 ATG9-GFP T119DExpresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag
114669pRS406 ATG9 stopGFPExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Antibodies from Article