Multiplexed deactivated CRISPR-Cas9 gene expression perturbations deter bacterial adaptation by inducing negative epistasis.
Otoupal PB, Cordell WT, Bachu V, Sitton MJ, Chatterjee A
Commun Biol. 2018 Sep 3;1:129. doi: 10.1038/s42003-018-0135-2. eCollection 2018.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114632 | pdCas9-omega | Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP |