Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths.
Warner JB 4th, Ruff KM, Tan PS, Lemke EA, Pappu RV, Lashuel HA
J Am Chem Soc. 2017 Oct 18;139(41):14456-14469. doi: 10.1021/jacs.7b06659. Epub 2017 Oct 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114601 | pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C | Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat |
| 114602 | pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C | Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat |
| 114603 | pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C | Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat |
| 114604 | pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C | Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat |
| 114605 | pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C | Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat |
| 114606 | pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C | Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat |
| 114608 | pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat |
| 114609 | pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat |
| 114610 | pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat |
| 114611 | pTWIN1-His6-Ssp-Htt2-90-15Q-P90C | Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat |
| 114612 | pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat |
| 114613 | pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C | Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat |
| 114614 | pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat |
| 114615 | pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C | Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat |
| 114617 | pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat |
| 114618 | pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat |
| 114619 | pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat |
| 114620 | pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat |
| 114621 | pTWIN1-His6-Ssp-Htt2-90-37Q-P90C | Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat |
| 114622 | pTWIN1-His6-Ssp-Httex1-43Q-A2C | Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat |
| 114623 | pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C | Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeat |
| 114624 | pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat |
| 114625 | pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C | Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat |
| 114626 | pTWIN1-His6-Ssp-Htt2-90-43Q-P90C | Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat |
| 114627 | pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeat |
| 114628 | pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat |
| 114629 | pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P70C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 49 polyQ repeat |
| 114630 | pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C | Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat |
| 114631 | pTWIN1-His6-Ssp-Htt2-90-49Q-P90C | Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat |