Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors.

Voyvodic PL, Pandi A, Koch M, Conejero I, Valjent E, Courtet P, Renard E, Faulon JL, Bonnet J
Nat Commun. 2019 Apr 12;10(1):1697. doi: 10.1038/s41467-019-09722-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
114597	pBEAST-BenR	"Strong constitutive expression of transcription factor, BenR, for cell-free expression"
114598	pBEAST-pBen-sfGFP	"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"
114599	pBEST-HipO	Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression
114600	pBEAST-J23101-CocE	"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"
116917	pBEAST-J23101-sfGFP	Strong constitutive expression of super-folder GFP for cell-free expression
122694	pBEAST-pBen-Luc	Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression
126575	pBEAST2-sfGFP	Strong constitutive expression of super-folder GFP for cell-free expression