Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins.
Van Geel O, Hartsuiker R, Gadella TWJ
Small GTPases. 2018 Sep 5:1-10. doi: 10.1080/21541248.2018.1507411.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114415 | iLID-eGFP-C2 | anchor iLID domain to plasma membrane with slower diffusion |
| 114416 | iLID-eGFP-2xC2 | anchor iLID domain to plasma membrane with slower diffusion |
| 114417 | iLID-eGFP-3xC2 | anchor iLID domain to plasma membrane with slower diffusion |
| 114418 | iLID-eGFP-2xC2-iLID | anchor iLID domain to plasma membrane with slower diffusion |
| 114419 | EB3-eGFP-2xC2-iLID | anchor iLID domain to plasma membrane with slower diffusion |
| 114420 | CRY2clust-eGFP-2xC2-iLID | anchor iLID domain to plasma membrane with slower diffusion |
| 114421 | mSc-sspB | Translocating to iLID domain |
| 114422 | sspB-mSc-sspB | Translocating to iLID domain |
| 114423 | 2xsspB-mSc-sspB | Translocating to iLID domain |
| 114424 | 2xsspB-mSc-sspB-Tiam1 | Translocating Tiam1 to iLID and activate Rac1 |
| 114425 | 2xsspB-mSc-sspB-ITSN1 | Translocating ITSN1 to iLID and activate Cdc42 |