Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase.
Cinesi C, Aeschbach L, Yang B, Dion V
Nat Commun. 2016 Nov 9;7:13272. doi: 10.1038/ncomms13272.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114384 | pPN10-gCAG | pPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAG |
| 114385 | pPN10-gCTG | pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG |
| 114386 | pPN10 | Empty gRNA |
| 114387 | pPN10-gDM1d | pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG |