MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme.
Lin D, O'Callaghan CA
Nucleic Acids Res. 2018 Jul 9. pii: 5050631. doi: 10.1093/nar/gky596.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114219 | pMXLC_pFa | POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a |
| 114220 | pMXLC_aFb | POC12356, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-b |
| 114221 | pMXLC_bFc | POC12357, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-c |
| 114222 | pMXLC_cFd | POC12358, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type c-d |
| 114223 | pMXLC_dFe | POC12359, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type d-e |
| 114224 | pMXLC_aFq | POC12362, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-q |
| 114225 | pMXLC_bFq | POC12363, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-q |
| 114226 | pMXLC_cFq | POC12364, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type c-q |
| 114227 | pMXLC_dFq | POC12365, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type d-q |
| 114228 | pMXLC_eFq | POC12366, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type e-q |
| 114229 | pMXLK_pFa | POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a |
| 114230 | pMXLK_aFb | POC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b |
| 114231 | pMXLK_bFc | POC12369, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-c |
| 114232 | pMXLK_cFd | POC12370, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-d |
| 114233 | pMXLK_dFe | POC12371, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-e |
| 114234 | pMXLK_aFq | POC12374, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-q |
| 114235 | pMXLK_bFq | POC12375, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-q |
| 114236 | pMXLK_cFq | POC12376, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-q |
| 114237 | pMXLK_dFq | POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q |
| 114238 | pMXLK_eFq | POC12378, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type e-q |
| 114239 | pMXLP_pFa | POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a |
| 114240 | pMXLP_aFb | POC12380, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type a-b |
| 114241 | pMXLP_bFc | POC12381, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type b-c |
| 114242 | pMXLP_cFd | POC12382, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type c-d |
| 114243 | pMXLP_dFe | POC12383, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type d-e |
| 114244 | pMXLP_aFq | POC12386, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type a-q |
| 114245 | pMXLP_bFq | POC12387, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type b-q |
| 114246 | pMXLP_cFq | POC12388, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type c-q |
| 114247 | pMXLP_dFq | POC12389, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type d-q |
| 114248 | pMXLP_eFq | POC12390, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type e-q |
| 114249 | pMXBC_pFa | POC12391, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a |
| 114250 | pMXBC_aFb | POC12392, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-b |
| 114251 | pMXBC_bFc | POC12393, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-c |
| 114252 | pMXBC_aFq | POC12394, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-q |
| 114253 | pMXBC_bFq | POC12395, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-q |
| 114254 | pMXBC_cFq | POC12396, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type c-q |
| 114255 | pMXBK_pFa | POC12397, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a |
| 114256 | pMXBK_aFb | POC12398, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b |
| 114257 | pMXBK_bFc | POC12399, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-c |
| 114258 | pMXBK_aFq | POC12400, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-q |
| 114259 | pMXBK_bFq | POC12401, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type b-q |
| 114260 | pMXBK_cFq | POC12402, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-q |
| 114261 | pMXBP_pFa | POC12403, MetClo assembly vector with F replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a |
| 114262 | pMXBP_aFb | POC12404, MetClo assembly vector with F replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type a-b |
| 114263 | pMXBP_bFc | POC12405, MetClo assembly vector with F replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type b-c |
| 114264 | pMXBP_aFq | POC12406, MetClo assembly vector with F replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type a-q |
| 114265 | pMXBP_bFq | POC12407, MetClo assembly vector with F replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type b-q |
| 114266 | pMXBP_cFq | POC12408, MetClo assembly vector with F replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type c-q |
| 114267 | DH10B-M.Osp807II | The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus. |
| 114268 | DH10B-M2.NmeMC58II | The strain expresses M2.NmeMC58II methylase and zeocin selection marker from the arsB locus. |
| 114269 | DH10B-M.XmnI | The strain expresses M.XmnI methylase and zeocin selection marker from the arsB locus. |