A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation.
Savell KE, Bach SV, Zipperly ME, Revanna JS, Goska NA, Tuscher JJ, Duke CG, Sultan FA, Burke JN, Williams D, Ianov L, Day JJ
eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-0495-18. doi: 10.1523/ENEURO.0495-18.2019. eCollection 2019 Jan-Feb.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114195 | lenti EF1a-FLAG-dCas9-VPR | Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter |
| 114196 | lenti SYN-FLAG-dCas9-VPR | Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter |
| 114197 | lenti CAG-FLAG-dCas9-VPR | Lentivirus compatible dCas9-VPR construct driven by the CAG promoter |
| 114199 | lenti U6-sgRNA/EF1a-mCherry | Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter |