ORAI1 channel gating and selectivity is differentially altered by natural mutations in the first or third transmembrane domain.

Bulla M, Gyimesi G, Kim JH, Bhardwaj R, Hediger MA, Frieden M, Demaurex N
J Physiol. 2018 Nov 1. doi: 10.1113/JP277079. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
114180	ORAI1-YFP V107M	ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).
114181	ORAI1-YFP T184M	ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).
114182	ORAI1-YFP L273D-L276D	ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.
114183	ORAI1-YFP V107M-L273D-L276D	ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
114184	ORAI1-YFP T184M-L273D-L276D	ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.