Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring.
Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus EP
Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 10.1038/nchembio.2448. Epub 2017 Aug 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114155 | pET28a-clbB | Expresses full-length ClbB |
| 114156 | pET28a-clbH | Expresses full-length ClbH |
| 114157 | pET28a-clbH-C-A2-PCP | Expresses ClbH-C-A2-PCP |
| 114158 | pET29b-clbH-C-A2-PCP S1524A | Expresses ClbH-C-A2-PCP S1524A |
| 114159 | pET29b-clbI S178A | Expresses ClbI S178A |