Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates.

Tillo SE, Xiong WH, Takahashi M, Miao S, Andrade AL, Fortin DA, Yang G, Qin M, Smoody BF, Stork PJS, Zhong H
Cell Rep. 2017 Apr 18;19(3):617-629. doi: 10.1016/j.celrep.2017.03.070. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
114113	pCDNA3-PKA-RIIβ-sREACh	Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.
114114	pCDNA3_PKA-CαN-mPAGFP	Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
114130	pCDNA3_nmPKA-CαN-mPAGFP	Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
114131	pCDNA3_nmPKA-Cα-mPAGFP	Mammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
114132	pCDNA3_altPKA-Cα-mEGFP	Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.
114133	pCDNA3_altPKA-Cα-mPAGFP	Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
114134	pCDNA3_PKA-Cα(S11A)-mPAGFP	Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
114135	pCDNA3_PKA-Cα(S11D)-mPAGFP	Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
114136	pEGFP_mEGFP-GluA1c	Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.
114138	pCAGGS_cyt-AKAR5	Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
114139	pCAGGS_m-AKAR5	Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
114140	pCAGGS_m-AKAR5’	Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM
114141	pCAGGS_AKAR5’	Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
114142	pEFU6-EGFP_shPKA-Cα	Expression of shRNA for knocking down endogenous PKA-Cα. EGFP is also expressed.
114143	pCDNA3_shRNA resistant PKA-Cα-mEGFP	Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
114144	pCDNA3_shRNA resistant nmPKA-Cα-mEGFP	The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.