A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B.
Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ
J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. Epub 2013 Apr 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114044 | pCDNA5-LAP-MPS1-WT | Expression of exogenous LAP tagged MPS1 WT |
| 114045 | pCDNA5-LAP-MPS1-KD | Expression of exogenous LAP tagged MPS1 Kinase-dead |
| 114046 | pCDNA5-LAP-MPS1-d60 | Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted) |
| 114047 | pCDNA5-LAP-MPS1-d200 | Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted) |
| 114048 | pCDNA5-LAP-MPS1-dTPR | Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted) |
| 114049 | pLacI-GFP-HEC1 WT | Expression of exogenous LacI-GFP-HEC1 WT |
| 114050 | pLacI-GFP-HEC1-d207 | Expression of exogenous LacI-GFP-HEC1 delta 207 |
| 114051 | pcDNA5-hygro-eGFP-HEC1-d207 | Expression of exogenous eGFP HEC1 delta 207 |