Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase.
Kuijt TE, Omerzu M, Saurin AT, Kops GJ
Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. Epub 2014 Apr 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114037 | pcDNA5-eYFP-FKBP-MAD1-WT | Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB) |
| 114038 | pcDNA5-eYFP-FKBP-MAD1-AA | Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB) |
| 114039 | MIS12-FRB-tagRFP | Kinetochore localised FRB tagged with FLAG and tagRFP |
| 114040 | MIS12-FRB-FLAG | Kinetochore localised FRB tagged with FLAG |