Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint.

Etemad B, Kuijt TE, Kops GJ
Nat Commun. 2015 Dec 1;6:8987. doi: 10.1038/ncomms9987. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
114021	pLV-CMV-tagRFP-Mad2	Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter
114022	pcDNA5-puro-eGFP-HEC1	Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines
114023	pcDNA5-puro-eGFP-HEC1-9A	Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines
114025	pcDNA5-puro-eGFP-HEC1-d80	Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines
114026	pcDNA5-hygro-eGFP-STOP-HEC1	Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.
114027	pcDNA5-hygro-eGFP-HEC1	Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines
114028	pcDNA5-hygro-eGFP-HEC1-9A	Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines
114029	pcDNA5-hygro-eGFP-HEC1-9D	Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines
114030	pcDNA5-hygro-eGFP-HEC1-d80	Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines
114031	pcDNA5-hygro-eGFP-HEC1-8A/S15D	Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines
114032	pcDNA5-hygro-eGFP-HEC1-8A/S55D	Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines