Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics.
Hulton CH, Costa EA, Shah NS, Quintanal-Villalonga A, Heller G, de Stanchina E, Rudin CM, Poirier JT
Nat Cancer. 2020 Mar;1(3):359-369. doi: 10.1038/s43018-020-0040-8. Epub 2020 Mar 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114010 | pSpCTRE-CD4 | Dox-inducible Cas9 expression with a CD4 selection marker |
| 114011 | sgTrack-Gateway | 3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA |
| 114012 | sgTrack-GFP | Empty sgRNA expression vector with co-expression of TurboGFP reporter |
| 114013 | sgTrack-mCherry | Empty sgRNA expression vector with co-expression of mCherry reporter |