A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9.

Cui L, Vigouroux A, Rousset F, Varet H, Khanna V, Bikard D
Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5. PubMed

Plasmids from Article

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ID	Plasmid	Purpose
116947	pOSIP-KL-mcherry	Integrating mCherry into lambda attB in E. coli chromosome.
115927	psgRNA Library	Genome-wide knockdown of E. coli genes
115926	AV04	Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome
115925	LC-E75	Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome
115924	LC-E18	Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome
114006	psgRNAc	express sgRNA, p15A, CRM
114005	psgRNA	express sgRNA. ColE1, Kan