A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors.
Geddes BA, Mendoza-Suarez MA, Poole PS
Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. eCollection 2018.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
113978 | pOGG001 | Level 0 module. nptII promoter for constitutive gene expression |
113979 | pOGG004 | Vector construction module. Level 1 golden gate cloning site module with LacZ reporter |
113980 | pOGG005 | Level 1 high copy number vector for E. coli expression |
113981 | pOGG006 | Golden Gate Level 2 cloning site, position 1 module for vector construction |
113982 | pOGG008 | Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors |
113983 | pOGG009 | Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors |
113984 | pOGG010 | Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors |
113985 | pOGG011 | Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors |
113986 | pOGG012 | Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors |
113987 | pOGG013 | Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3 |
113988 | pOGG014 | Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4 |
113989 | pOGG015 | Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5 |
113990 | pOGG016 | Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6 |
113991 | pOGG024 | Level 1 golden gate cloning vector for medium copy number free-living expression |
113992 | pOGG026 | Level 1 golden gate cloning vector for low copy number stable environmental gene expression |
113993 | pOGG030 | Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification |
113994 | pOGG031 | Level 0 module. IPTG promoter for inducible expression |
113995 | pOGG037 | SC module. Super-folder GFP green fluorescence reporter |
113996 | pOGG039 | SC module. Terminator for T7RNAP |
113997 | pOGG041 | Level 0 module. Taurine-inducible promoter for Alphaproteobacteria |
113998 | pOGG042 | Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors |
113999 | pOGG050 | SC module. Thermostable beta-galactosidase reporter |
114000 | pOGG082 | Level 0 module. NifH promoter for nodule-specific expression |
114001 | pOGG083 | SC module. Beta-glucosidase reporter |
114002 | pOGG216 | Level 2 golden gate cloning vector for medium copy number free-living expression |
115503 | pOGG202 | Clone of a level 1 unit which encodes IPTG-inducible sfGFP |
115504 | pOGG203 | Clone of a level 1 unit which encodes a constitutively-expressed mCherry |
115505 | pOPS0253 | Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA. |
115506 | pOPS0254 | Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB |
115507 | pOPS0314 | Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB |
115508 | pOPS0359 | Reporter Plasmid. Taurine-inducible expression of GFP |
115509 | pOPS0377 | Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP |
115510 | pOPS0379 | Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP |
115511 | pOPS0750 | Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA. |