Optical control of membrane tethering and interorganellar communication at nanoscales.
He L, Jing J, Zhu L, Tan P, Ma G, Zhang Q, Nguyen NT, Wang J, Zhou Y, Huang Y
Chem Sci. 2017 Aug 1;8(8):5275-5281. doi: 10.1039/c7sc01115f. Epub 2017 May 31.
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PubMed
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
113934 | GFP-LiMETER-v2 | Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP |
113935 | mCherry-OptoPB | Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions |