A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains.
Wijsman M, Swiat MA, Marques WL, Hettinga JK, van den Broek M, Torre Cortes P, Mans R, Pronk JT, Daran JM, Daran-Lapujade P
FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5114578. doi: 10.1093/femsyr/foy107.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113870 | pUDR211 | expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively |
| 113871 | pURD214 | expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively |
| 113872 | pUDR217 | expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively |
| 113873 | pUDR220 | expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively |
| 113874 | pUDR295 | expression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectively |
| 113875 | pUDR418 | expression of double Cas9 programming sgRNA11 targetting STL1 |