Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway.
Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113708 | pMuLE_ENTR_CBF-tdTomato_L3-L2 | Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector. |
| 113712 | pMuLE_ENTR_12GLI-FLuc_R4-R3 | Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector. |
| 113715 | EF.hICN1 | Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed. |
| 113862 | pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc | Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation. |
| 113864 | pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3 | Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector. |
| 113865 | pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato | Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation. |
| 113866 | pMuLE_ENTR_TOP-iRFP_L5-L4 | Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector. |
| 113867 | pMuLE_ENTR_TOP-NLuc1.1_L5-L4 | Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector. |
| 113868 | pMuLE_ENTR_CBF-GLuc_L3-L2 | Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector. |