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Entropic Control of Receptor Recycling Using Engineered Ligands.

DeGroot ACM, Busch DJ, Hayden CC, Mihelic SA, Alpar AT, Behar M, Stachowiak JC
Biophys J. 2018 Mar 27;114(6):1377-1388. doi: 10.1016/j.bpj.2018.01.036. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113552pRSET his-mCherryFor bacterial expression and purification of mCherry
113553pRSET his-mRFPFor bacterial expression and purification of mRFP
113554TfRΔEctoR1-eGFP A206KTransmembrane model receptor to probe receptor internalization via clathrin mediated pathway
113555TfRΔEctoR1-mRFP-GFPnbTransmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Antibodies from Article