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Crosstalk between the RNA Methylation and Histone-Binding Activities of MePCE Regulates P-TEFb Activation on Chromatin.

Shelton SB, Shah NM, Abell NS, Devanathan SK, Mercado M, Xhemalce B
Cell Rep. 2018 Feb 6;22(6):1374-1383. doi: 10.1016/j.celrep.2018.01.028. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113535pSUPER.retro.puro-shNCControl retroviral vector.
113536pSUPER.retro.puro-shMePCERetroviral vector designed to knock down MePCE.
113537pSUPER.retro.puro-shLARP7Retroviral vector designed to knock down LARP7.
113538pSUPER.retro.puro-shMePCE_shLARP7Retroviral vector designed to knock down MePCE and LARP7 simultaneously.
113539pGEX-5X-1-Full-Length-MePCEProduces an N-terminal GST-tagged full length MePCE protein.
113541pGEX-5X-1_MePCE_aa1-400Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.
113542pGEX-5X-1_MePCE_aa400-689Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.
113543pGEX-5X-1_MePCE_aa432-689Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.
113544pGEX-5X-1-MePCE_aa470-689Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.
113545pGEX-5X-1-LARP7Produces an N-terminal GST-tagged full length LARP7 protein.
113547pcDNA5/FRT/TO/intron/GFPMammalian expression plasmid that can be used with the Flp-In T-REx system.
113548pcDNA5/FRT/TO/intron/FlagCterMammalian expression plasmid that can be used with the Flp-In T-REx system.
113549pcDNA5/FRT/TO-Intron-CterFlag-MePCEMammalian expression plasmid that can be used with the Flp-In T-REx system.

Antibodies from Article