Addgene: LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.

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Erich Wanker
Trepte et al

LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.

Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau K, Schnoegl S, Garner CC, Wanker EE
Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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Search table:

ID	Plasmid	Purpose
113459	pcDNA3.1 PA-mCit-BAD	ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)
113458	pcDNA3.1 NL-BCL2L1	NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)
113457	pLenti GW-mCit-PA	Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions
113456	pLenti PA-mCit-GW	Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions
113455	pLenti GW-NL-myc	Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions
113454	pLenti myc-NL-GW	Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions
113453	pLenti PA-NL	Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)
113452	pLenti PA-mCit-NL	Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)
113451	pLenti PA-mCit	Lentiviral ProteinA-mCitrine expression vector
113450	pLenti NL	Lentiviral NanoLuc control expression vector

Antibodies from Article