CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.
Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA
Science. 2018 Apr 27;360(6387):436-439. doi: 10.1126/science.aar6245. Epub 2018 Feb 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113430 | pMBP-AsCas12a | Expresses 10xHis-MBP fused to AsCas12a |
| 113431 | pMBP-LbCas12a | Expresses 10xHis-MBP fused to LbCas12a |
| 113432 | pMBP-FnCas12a | Expresses 10xHis-MBP fused to FnCas12a |
| 113433 | pMBP-AaCas12b | Expresses Expresses 10xHis-MBP fused to AaCas12b |
| 113517 | pMBP-LbCas12a D832A | Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease |
| 113518 | pMBP-LbCas12a H759A | Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing |