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A proximity-tagging system to identify membrane protein-protein interactions.

Liu Q, Zheng J, Sun W, Huo Y, Zhang L, Hao P, Wang H, Zhuang M
Nat Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 10.1038/s41592-018-0100-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113399pEF6a-BCCP-PupEExpresses BCCP-PupE in mammalian cell
113400pEF6a-CD28-PafAExpresses CD28-PafA in mammalian cell
113401pEF6a-FKBP-CD28Expresses FKBP-CD28 in mammalian cell
113402pGEX6p-1-PafA-XIAPExpresses PafA-XIAP in bacteria
113403pGEX6p-1-BCCP-PupEExpresses BCCP-PupE in bacteria
113404pGEX6p-1-FRB-PafAExpresses FRB-PafA in bacteria
113405pLVX-TRE3G-BCCP-PupE-IRES-BFPExpress inducible BCCP-PupE in mammalian cell

Antibodies from Article