Pooled extracellular receptor-ligand interaction screening using CRISPR activation.
Chong ZS, Ohnishi S, Yusa K, Wright GJ
Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113341 | pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA | Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins |
| 113342 | pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA | Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins |
| 113343 | pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA | Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins |
| 113344 | pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA | Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins |
| 113345 | Membrane protein gRNA library | Contains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAs |