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Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria.

Dong C, Fontana J, Patel A, Carothers JM, Zalatan JG
Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113314pCD005W108 scRNA
113315pCD061W108 scRNA.b1
113316pCD175dCas9, MCP-AsiA, rpoD_F563Y
113317pCD185dCas9, MCP-SoxS_R93A
113318pCD227AraC-pBAD-dCas9, MCP-SoxS_R93A
113319pCD294.9dCas9, PCP-SoxS_R93A, J109 scRNA.b1
113320pCD296.7dCas9, MCP-TetD, J107 scRNA.b1
113321pCD315pBAD-W108 scRNA.b1
113322pJF076SaJ1-mRFP1
113323pJF093Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A
113324pJF104BpTet-dCas9, pTet-MCP-SoxS_R93A
113325pJF121pTet-W108 scRNA.b2
113326pJF131BpTet-W108 scRNA.b2, pTet-RR2-sgRNA

Antibodies from Article