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Rational design of a split-Cas9 enzyme complex.

Wright AV, Sternberg SH, Taylor DW, Staahl BT, Bardales JA, Kornfeld JE, Doudna JA
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):2984-9. doi: 10.1073/pnas.1501698112. Epub 2015 Feb 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113255pAW-AlphaLobeE. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Antibodies from Article