Adam Karpf Lab: CRISPR-mediated insertion of DR-GFP into the AAVS1 locus
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113193 | AAVS1-DRGFP | DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin. |
| 113194 | PX458-AAVS1 | Encodes gRNA for human AAVS1 |