Adam Karpf Lab: CRISPR-mediated insertion of DR-GFP into the AAVS1 locus

Unpublished

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ID	Plasmid	Purpose
113194	PX458-AAVS1	Encodes gRNA for human AAVS1
113193	AAVS1-DRGFP	DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.