Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells.
Severi F, Conticello SG
RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112857 | rat APOBEC1-EGFP | plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein |
| 112858 | rat APOBEC1 | plasmid for expression of rat APOBEC1 in mammalian cells |
| 112859 | pEGFP mCherry-ApoB-EGFP | Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP |