Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.
Wang T, Guan C, Guo J, Liu B, Wu Y, Xie Z, Zhang C, Xing XH
Nat Commun. 2018 Jun 26;9(1):2475. doi: 10.1038/s41467-018-04899-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113134 | E.coli_GW-sgRNA_Lib1 | Genome-wide gRNA library for E. coli, sub1 |
| 113135 | E.coli_GW-sgRNA_Lib2 | Genome-wide gRNA library for E. coli, sub2 |
| 113136 | E.coli_GW-sgRNA_Lib3 | Genome-wide gRNA library for E. coli, sub3 |
| 113137 | E.coli_GW-sgRNA_Lib4 | Genome-wide gRNA library for E. coli, sub4 |
| 113146 | E.coli_NC_sgRNA_lib | Genome-wide gRNA library for E. coli, non-targeting control |
| 113149 | pdCas9-J23111 | Plasmid constitutively expressing dCas9 protein |