Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research.
Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T
Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113081 | T444T | Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites. |
| 113082 | T777T | Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence. |
| 113083 | Ccas9LacZ | Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences. |