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CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window.

Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE
Nature. 2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 10.1038/s41586-018-0384-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113077pEvolvR-enCas9-PolI3M-TBDExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.
113078pEvolvR-enCas9-PolI5MExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Antibodies from Article