CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window.
Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE
Nature. 2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 10.1038/s41586-018-0384-8.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
113077 | pEvolvR-enCas9-PolI3M-TBD | Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning. |
113078 | pEvolvR-enCas9-PolI5M | Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning. |