Pooled extracellular receptor-ligand interaction screening using CRISPR activation.
Chong ZS, Ohnishi S, Yusa K, Wright GJ
Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112919 | pKLV2-EF1adCas9VP64T2ABsd-W | Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter |
| 112920 | pKLV2-EF1aMS2p65HSF1hyg-W | Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter |
| 112921 | pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA | Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker |
| 112922 | pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA | Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker |
| 112923 | pPB-R1R2-EM7neoPheS | piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection) |
| 112924 | pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA | piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker |
| 112925 | pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W | Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP |
| 112926 | pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W | CRISPR-a reporter assay lentiviral vector (empty gRNA control) |
| 112927 | pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W | CRISPR-a reporter assay lentiviral vector (gTetO) |