Pooled extracellular receptor-ligand interaction screening using CRISPR activation.

Chong ZS, Ohnishi S, Yusa K, Wright GJ
Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
112927	pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W	CRISPR-a reporter assay lentiviral vector (gTetO)
112926	pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W	CRISPR-a reporter assay lentiviral vector (empty gRNA control)
112925	pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W	Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP
112924	pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA	piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker
112923	pPB-R1R2-EM7neoPheS	piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)
112922	pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA	Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker
112921	pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA	Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker
112920	pKLV2-EF1aMS2p65HSF1hyg-W	Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter
112919	pKLV2-EF1adCas9VP64T2ABsd-W	Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter