Robust CRISPR/Cas9-Mediated Tissue Specific Mutagenesis Reveals Gene Redundancy and Perdurance in Drosophila.
Poe AR, Wang B, Sapar ML, Ji H, Li K, Onabajo T, Fazliyeva R, Gibbs M, Qiu Y, Hu Y, Han C
Genetics. 2018 Nov 30. pii: genetics.118.301736. doi: 10.1534/genetics.118.301736.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112808 | pAC-U61-SapI | gRNA cloning vector for expressing 1-3 gRNAs in Drosophila |
| 112809 | pTGC-U6.2 | PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment |
| 112810 | pTGC-U6.3 | PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment |
| 112811 | pAC-U63-tgRNA-Rev | gRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in Drosophila |
| 112812 | pMGC | PCR template vector for amplifying gRNA(F+E)-OstRNA fragment |
| 112813 | pDEST-HemmarR2 | gateway cloning destination vector for expressing enhancer driven CD4-tdTomato |
| 112814 | pDEST-HemmarG2 | gateway cloning destination vector for expressing enhancer driven CD4-tdGFP |
| 112815 | pDEST-HemmarI2 | gateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1 |
| 121657 | pDEST-APIC-Cas9 | gateway cloning destination vector for expressing enhancer driven Cas9 |