How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration.
Xiao Y, Ng S, Nam KH, Ke A
Nature. 2017 Oct 5;550(7674):137-141. doi: 10.1038/nature24020. Epub 2017 Sep 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112793 | His6-SUMO-pET28a-EfaCas1 | To express enterococcus faecalis Cas1 protein in E. coli |
| 112794 | His6-Twin-Strep-SUMO-pET28a-EfaCas2 | To express enterococcus faecalis Cas2 protein in E. coli |