A nutrient-induced affinity switch controls mTORC1 activation by its Rag GTPase-Ragulator lysosomal scaffold.

Lawrence RE, Cho KF, Rappold R, Thrun A, Tofaute M, Kim DJ, Moldavski O, Hurley JH, Zoncu R
Nat Cell Biol. 2018 Sep;20(9):1052-1063. doi: 10.1038/s41556-018-0148-6. Epub 2018 Jul 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
112774	pRK5 Flag RagC(GD)/RagB(CTD) chimera	express Flag-RagC (G domain) fused with with RagB (C terminal domain)
112773	pRK5 Flag RagB(GD)/RagC(CTD) chimera	express Flag-RagB (Gdomain) fused with RagC (C terminal domain)
112772	pRK5 Flag RagC (GD)	express Flag-RagC (G domain only)
112771	pRK5 Flag RagC (CTD)	express Flag-RagC (C terminal domain only)
112770	pRK5 Flag RagB (GD)	express Flag-RagB (G domain only)
112769	pRK5 Flag RagB (CTD)	express Flag-RagB (C terminal domain only)
112768	pRK5 GFP RagC (CTD)	express GFP-RagC (C terminal domain only)
112767	pRK5 GFP RagC (GD)	express GFP-RagC (G domain only)
112766	pRK5 GFP RagB (CTD)	express GFP-RagB (C terminal domain only)
112765	pRK5 GFP RagB (GD)	express GFP-RagB (G domain only)