A nutrient-induced affinity switch controls mTORC1 activation by its Rag GTPase-Ragulator lysosomal scaffold.
Lawrence RE, Cho KF, Rappold R, Thrun A, Tofaute M, Kim DJ, Moldavski O, Hurley JH, Zoncu R
Nat Cell Biol. 2018 Sep;20(9):1052-1063. doi: 10.1038/s41556-018-0148-6. Epub 2018 Jul 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112740 | pLJM1 FRB-myc-RagA | express FRB-myc-RagA |
| 112741 | pLJM1 FRB-myc-RagC | express FRB-myc-RagC |
| 112742 | pLJM1 TMEM192-Flag | express pLJM1 TMEM192-Flag |
| 112743 | pLJM1 TMEM192-Flag-FKBP | express TMEM192-Flag-FKBP |
| 112744 | pLJM1 Raptor GFP | express Raptor-GFP |
| 112749 | pRK5 GFP RagB Q99L | express GFP-RagB (Q99L mutant) |
| 112750 | pRK5 GST MetaP2 | express HA-GST-FRB-MetaP2 |
| 112752 | pRK5 Flag RagC (D181N) | express Flag-RagC (D181N mutant) |
| 112753 | pRK5 Halo RagB | express Halo-RagB |
| 112754 | pEGFP-N1 Raptor Halo | express Raptor-Halo |
| 112755 | pRK5 Flag RagB | express Flag-RagB |
| 112756 | pLVX Flag RagC WT | express Flag-RagC |
| 112758 | pLVX Flag RagC T90N | express Flag-RagC (T90N mutant) |
| 112759 | pLVX Flag RagC W115R | express Flag-RagC (W115R mutant) |
| 112760 | pLJM1 Flag GFP 4E-BP1 Omp25 | express GFP-4EBP1-Omp25 |
| 112761 | pLJM1 Flag GFP 4E-BP1 Rheb15 | express GFP-4EBP1-Rheb15 |
| 112762 | pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX | express GFP-4EBP1-Rheb15(CAAX mutation) |
| 112764 | pRK5 Myc Rheb Omp25 | express myc-Rheb-Omp25 |
| 112765 | pRK5 GFP RagB (GD) | express GFP-RagB (G domain only) |
| 112766 | pRK5 GFP RagB (CTD) | express GFP-RagB (C terminal domain only) |
| 112767 | pRK5 GFP RagC (GD) | express GFP-RagC (G domain only) |
| 112768 | pRK5 GFP RagC (CTD) | express GFP-RagC (C terminal domain only) |
| 112769 | pRK5 Flag RagB (CTD) | express Flag-RagB (C terminal domain only) |
| 112770 | pRK5 Flag RagB (GD) | express Flag-RagB (G domain only) |
| 112771 | pRK5 Flag RagC (CTD) | express Flag-RagC (C terminal domain only) |
| 112772 | pRK5 Flag RagC (GD) | express Flag-RagC (G domain only) |
| 112773 | pRK5 Flag RagB(GD)/RagC(CTD) chimera | express Flag-RagB (Gdomain) fused with RagC (C terminal domain) |
| 112774 | pRK5 Flag RagC(GD)/RagB(CTD) chimera | express Flag-RagC (G domain) fused with with RagB (C terminal domain) |