Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition.
Ordovas L, Boon R, Pistoni M, Chen Y, Wolfs E, Guo W, Sambathkumar R, Bobis-Wozowicz S, Helsen N, Vanhove J, Berckmans P, Cai Q, Vanuytsel K, Eggermont K, Vanslembrouck V, Schmidt BZ, Raitano S, Van Den Bosch L, Nahmias Y, Cathomen T, Struys T, Verfaillie CM
Stem Cell Reports. 2015 Nov 10;5(5):918-931. doi: 10.1016/j.stemcr.2015.09.004. Epub 2015 Oct 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112666 | pZ:F3-CAGGS GPHTK-F | Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus |
| 112667 | pZ:F3-P CAGGS tdTPH-F | Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest. |
| 112668 | pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2 | Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest. |
| 112669 | pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2 | Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators. |
| 115518 | pZ P (cHS4)X4 OCT4p eGFP SV40pA v2 | Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators. |