Dissection of GTPase-activating proteins reveals functional asymmetry in the COPI coat of budding yeast.
Arakel EC, Huranova M, Estrada AF, Rau EM, Spang A, Schwappach B
J Cell Sci. 2019 Aug 29;132(16). pii: jcs.232124. doi: 10.1242/jcs.232124.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112646 | p415 Gcs1 | Expression S.cerevisiae |
| 112647 | p415 Gcs1 R54K | Expression S.cerevisiae |
| 112648 | p415 Gcs1 R54K L246D | Expression S.cerevisiae |
| 112649 | p415 Gcs1 R54K AxxA | Expression S.cerevisiae |
| 112650 | p415 Gcs1 Δ3xF AxxA | Expression S.cerevisiae |
| 112651 | p415 Glo3 | Expression S.cerevisiae |
| 112652 | p415 Glo3 R59K | Expression S.cerevisiae |
| 112653 | p415 Glo3 R59K ΔC | Expression S.cerevisiae |
| 112654 | p415 Glo3 R59K ΔGRM-ΔC | Expression S.cerevisiae |
| 112655 | p415 Glo3 R59K Δ2x+ve | Expression S.cerevisiae |
| 112656 | p415 Glo3 R59K S389,398 A | Expression S.cerevisiae |
| 112657 | p415 Glo3 R59K S389,398 D | Expression S.cerevisiae |
| 112658 | p415 GFP-Glo3 | Expression S.cerevisiae |
| 112659 | p415 GFP-Glo3 ΔGRM-ΔC | Expression S.cerevisiae |
| 112660 | p415 GFP-Glo3 S389,398 A | Expression S.cerevisiae |
| 112661 | p415 GFP-Glo3 S389,398 D | Expression S.cerevisiae |
| 112662 | p415 GFP-Glo3 Δ2x+ve | Expression S.cerevisiae |
| 123286 | A102-GAP-BoCCS-GRM | Expression of Glo3 ΔAmpH (1-459) in E.Coli |
| 123287 | A102-GAP-BoCCS | Expression of truncated Glo3 (1-375) in E.Coli |
| 123288 | A102-BoCCS-GRM | Expression of truncated Glo3 (137-459) in E.Coli |
| 123289 | A102-Par-BoCCS-GRM | Expression of truncated Glo3 (296-459) in E.Coli |
| 123290 | A102-BoCCS | Expression of truncated Glo3 (137-375) in E.Coli |
| 123291 | A102-GRM | Expression of truncated Glo3 (373-459) in E.Coli |
| 129484 | A102 ΔAmpH Δ2x+ve | Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli |
| 129485 | p415 Glo3 S389,398 A | Expression in S. cerevisiae |
| 129486 | p415 Glo3 S389,398 D | Expression in S. cerevisiae |
| 129487 | p415 Met25 Glo3 ΔGRM-ΔC | Expression in S. cerevisiae |