Programmed DNA destruction by miniature CRISPR-Cas14 enzymes.
Harrington LB, Burstein D, Chen JS, Paez-Espino D, Ma E, Witte IP, Cofsky JC, Kyrpides NC, Banfield JF, Doudna JA
Science. 2018 Oct 18. pii: science.aav4294. doi: 10.1126/science.aav4294.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112500 | pLBH531_MBP-Cas14a1 expression | Expresses MBP fused to Cas14a1 |
| 112501 | pLBH545_Tet-Cas14a1_Locus | Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter |
| 112502 | pLBH559_Tet-HisCas14a1Locus | Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter |
| 112503 | pLBH546_Tet-Cas14b1Locus | Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter |
| 112504 | pLBH547_Tet-Cas14b2Locus | Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter |
| 112505 | pLBH532_MBP-Cas14b1 expression | Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter |
| 112506 | pLBH533_MBP-Cas14b2 expression | Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter |