Quantitative Analysis Reveals that Actin and Src-Family Kinases Regulate Nuclear YAP1 and Its Export.
Ege N, Dowbaj AM, Jiang M, Howell M, Hooper S, Foster C, Jenkins RP, Sahai E
Cell Syst. 2018 Jun 27;6(6):692-708.e13. doi: 10.1016/j.cels.2018.05.006. Epub 2018 Jun 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112284 | pLL3.7-EF-EYFP-YAP1_WT-PolyA | Fluorescently labels WT human YAP1-2gamma isoform (504 aa) |
| 112285 | pLL3.7-EF-EYFP-YAP1_5SA-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms) |
| 112286 | pLL3.7-EF-EYFP-YAP1_S94A-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant |
| 112287 | pLL3.7-EF-EYFP-YAP1_Y357F-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform |
| 112288 | pLL3.7-EF-EYFP-YAP1_Y3F-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform |
| 112289 | pLL3.7-EF-EYFP-YAP1_delta5C-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein |
| 112290 | pLL3.7-EF-EYFP-YAP1_WW-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDA |
| 112291 | pLL3.7-EF-EYFP-YAP1_R89A-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant |
| 112292 | pLL3.7-EF-EYFP-YAP1_L91A-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant |
| 112293 | pLL3.7-EF-EYFP-YAP1_F95A-PolyA | Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant |