Wide-area all-optical neurophysiology in acute brain slices.
Farhi SL, Parot VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou S, Jun Kim J, Campbell RE, Cox DD, Cohen AE
J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 10.1523/JNEUROSCI.0168-19.2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102995 | pAAV-synp-F-H2B-jRGECO1a | Expresses histone fused jRGECO1a under the synapsin promoter |
| 112278 | SLF155: AAV-syn-DO-H2B-jRGECO1a | For AAV based expression of nuclear jRGECO1a in neurons in Cre-negative neurons with FLEx switch |
| 112280 | SLF045: AAV-hSyn-eTsChR-GFP | AAV based broad expression of eTsChR in neurons |
| 112281 | SLF091: Lenti-CaMKIIa-eTsChR-eGFP | For lentiviral or direct expression of eTsChR in excitatory neurons |
| 112282 | SLF060: Lenti-CaMKIIa-jRGECO1a | For lentiviral or direct expression of cytosolic jRGECO1a in excitatory neurons |
| 124775 | SLF063: Lenti-fCAG-jRGECO1a | For general lentiviral or direct expression of cytosolic jRGECO1a |
| 124776 | SL016: AAV-CAG-FLEX-CheRiff-GFP | For AAV based expression of CheRiff in Cre-Positive cells |