iKA-CRISPR hESCs for inducible and multiplex orthogonal gene knockout and activation.

Ma S, Lv J, Sun J, Tang P, Li H, Zhou H, Zhang Z, Lin Y, Rong Z
FEBS Lett. 2018 Jun 5. doi: 10.1002/1873-3468.13127. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
112261	Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1	Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.
112262	pU6-EF-gAAVS1	Express g-AAVS1