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Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.

Waldman AJ, Balskus EP
J Org Chem. 2018 May 29. doi: 10.1021/acs.joc.8b00367. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
112238pET-MBP-creMpET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM
112239pET-MBP-CreM_E352ApET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM with E352A mutation
112240pUWL201PW-creM-N-His6Streptomyces/E.coli shuttle expression vector encoding an N-terminal N-His6 tagged CreM
112241pUWL201PW-creDEStreptomyces/E.coli shuttle expression vector encoding creD and creE
112242pUWL201PW-creDEMStreptomyces/E.coli shuttle expression vector encoding creD, creE, creM

Antibodies from Article