Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion.
Luo J, Lu L, Gu Y, Huang R, Gui L, Li S, Qi X, Zheng W, Chao T, Zheng Q, Liang Y, Zhang L
J Biotechnol. 2018 Jun 7;281:11-20. doi: 10.1016/j.jbiotec.2018.06.308.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
112219 | pX458-DsRed2 | Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2 |
112220 | pX458-ECFP | Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP |