Phase transitions in the assembly of multivalent signalling proteins.

Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK
Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
112086	pMal_Abl-PRM 3R	Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
112087	pMAL-Abl-PRM 4R	Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.
112088	pMal-Abl-PRM 5R	Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.
112089	pMAL-SH3(2)-5R	Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
112090	pGEX SH3(2)-4R	Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.
112091	pGEX SH3(2)-3R	Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.
112154	mCherry-SH3(5R)	Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.
112155	eGFP-PRM-5R	Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.
112156	eGFP-PRM-3R	Mammalian eGFP expression plasmid for PRM-3R.